6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)

製品の概要

  • 製品名
    6-Phosphofructokinase Activity Assay Kit (Colorimetric)
  • サンプルの種類
    Cell Lysate, Tissue Lysate
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    < 1 mU/well
  • 検出範囲
    2 nmol/well - 10 nmol/well
  • 全工程の試験時間
    1h 30m
  • 種交差性
    交差種: Other species, Mammals
  • 製品の概要

    In Abcam's 6-Phosphofructokinase Activity Assay kit (ab155898), PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

  • 特記事項

    Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy.

製品の特性

画像

  • PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

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ab155898 has not yet been referenced specifically in any publications.

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