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RabMAb

Anti-5HT2C Receptor 抗体 [EPR6487] (ab133570)

製品の概要

  • 製品名
    Anti-5HT2C Receptor antibody [EPR6487]
    5HT2C Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6487] to 5HT2C Receptor
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human 5HT2C Receptor aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P28335
    (Peptide available as ab170038)

  • ポジティブ・コントロール
    • SH-SY5Y, BXPC3, MOLT4, mouse kidney and rat kidney lysates.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab133570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
  • ターゲット情報

    • 機能
      This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    • 配列類似性
      Belongs to the G-protein coupled receptor 1 family.
    • ドメイン
      The PDZ domain-binding motif is involved in the interaction with MPDZ.
    • 翻訳後修飾
      N-glycosylated.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5 Hydroxytryptamine 2C receptor antibody
      • 5-HT-1C antibody
      • 5-ht-1c receptor antibody
      • 5-HT-2C antibody
      • 5-HT1C antibody
      • 5-HT2C antibody
      • 5-HTR2C antibody
      • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
      • 5-hydroxytryptamine receptor 1C antibody
      • 5-hydroxytryptamine receptor 2C antibody
      • 5HT1C antibody
      • 5HT2C antibody
      • 5HT2C_HUMAN antibody
      • 5HTR2C antibody
      • 5Hydroxytryptamine 2C receptor antibody
      • Htr1c antibody
      • HTR2C antibody
      • serotonin 1c receptor antibody
      • serotonin 2c receptor antibody
      • Serotonin 5-HT-2C receptor antibody
      • Serotonin receptor 2C antibody
      see all

    画像

    • All lanes : Anti-5HT2C Receptor antibody [EPR6487] (ab133570) at 1/1000 dilution

      Lane 1 : Rat brain whole cell lysate
      Lane 2 : Rat brain whole cell lysate with blocking peptide

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 52 kDa
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 minutes

      See Abreview

    • All lanes : Anti-5HT2C Receptor antibody [EPR6487] (ab133570) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : BXPC3 cell lysate
      Lane 3 : MOLT4 cell lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 52 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    参考文献

    This product has been referenced in:
    • Ji G  et al. 5-HT2CReceptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors. J Neurosci 37:1378-1393 (2017). Read more (PubMed: 28011743) »

    See 1 Publication for this product

    レビューと Q&A

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 06 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Dec 04 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brains 25um sections)
    Specification
    brains 25um sections
    Fixative
    formalin
    Permeabilization
    Yes - 1xPBT
    Blocking step
    BSA as blocking agent for 11 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
    Username

    Mr. Luke Burke

    Verified customer

    投稿 Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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