Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-5HT1B Receptor 抗体 (ab13896)

Reviews (3)Q&A (6)References (17)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)
  • Western blot - Anti-5HT1B Receptor antibody (ab13896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)

Key features and details

  • Rabbit polyclonal to 5HT1B Receptor
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-5HT1B Receptor antibody
    5HT1B Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to 5HT1B Receptor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Rat 5HT1B Receptor. 8-26(CAPPPPATSQTGVPLANLS) and 263-278(VTSINSRVPEVPSESG)
    Database link: P28564

  • ポジティブ･コントロール

    • WB: Human brain lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13896の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa (predicted molecular weight: 47 kDa).
IHC-P (2)
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr (1)
Use at an assay dependent concentration. PubMed: 24642693
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa (predicted molecular weight: 47 kDa).
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration. PubMed: 24642693

ターゲット情報

  • 機能

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 翻訳後修飾

    Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
    Palmitoylated.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 5 HT1DB antibody
    • 5 hydroxytryptamine receptor 1B antibody
    • 5-HT-1B antibody
    • 5-HT-1D-beta antibody
    • 5-HT1B antibody
    • 5-hydroxytryptamine receptor 1B antibody
    • 5HT1B_HUMAN antibody
    • Htr1b antibody
    • HTR1D2 antibody
    • HTR1DB antibody
    • S12 antibody
    • Serotonin 1D beta receptor antibody
    • Serotonin receptor 1B antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)This image is courtesy of an anonymous Abreview

    ab13896 staining 5HT1B in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBFand blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-5HT1B Receptor antibody (ab13896)
    Western blot - Anti-5HT1B Receptor antibody (ab13896)
    Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected. Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT1B Receptor antibody (ab13896)

    ab13896 (4µg/ml) staining 5HT1B in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane compartment of the mature seminiferous tubules and possible interstitial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

参考文献 (17)

ab13896 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13896 は 17 報の論文で使用されています。

  • Pu ZH  et al. Alkaloids from the rhizomes of Ligusticum striatum exert antimigraine effects through regulating 5-HT1B receptor and c-Jun. J Ethnopharmacol 237:39-46 (2019). PubMed: 30880256
  • Frederiksen SD  et al. Expression of Pituitary Adenylate Cyclase-activating Peptide, Calcitonin Gene-related Peptide and Headache Targets in the Trigeminal Ganglia of Rats and Humans. Neuroscience 393:319-332 (2018). PubMed: 30336190
  • Han X  et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . PubMed: 29524471
  • Li Y  et al. Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nat Med 23:733-741 (2017). IHC ; Rat . PubMed: 28459438
  • Steinberg A  et al. Expression of messenger molecules and receptors in rat and human sphenopalatine ganglion indicating therapeutic targets. J Headache Pain 17:78 (2016). PubMed: 27587062
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-9 of 9 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5HT1B Receptor antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Jan 17 2022

Immunohistochemistry (Frozen sections) abreview for Anti-5HT1B Receptor antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, prefrontal cortex and caudate putamen)
Permeabilization
Yes - Triton-X 100
Specification
Brain, prefrontal cortex and caudate putamen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
At excision, the cerebellum was removed, the brain was divided in hemispheres and frozon in isopentane cooled on dry ice. Before IHC, sections were postfixated for 5 min in 96 % EtOH
Read More

Abcam user community

Verified customer

投稿 Oct 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5HT1B Receptor antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
10% NBF
Read More

Abcam user community

Verified customer

投稿 Dec 16 2013

Question

I used the recommended dilution by abcam 1 microgram per ml for (one up to over night incubation! time course), secondary antibody 1:5000 for 1 and 2 hrs..I wrote some other details in my previous email.I would like to know at what secondary antibody conditions( dilution and incubation time) it work for you guys...
thank you very much
please send me the detailed Secondary antibody conditions (diluent/dilution, incubation time/temperature) I tried the recommended primary antibody dilution I microgram per ml(1 hr to overnight incubation) and secondary 1:5000 RT for 1 hr, 2 hr...it is not working. I use SHySy5 cell line...and this protein is expressed in large amount as per litreature and our previous results..

Read More
Answer

Thank you for your response.

The information provided in the two e-mails is very limited. In the Lab, this antibody has been tested and characterized for Western blot application on human brain tissue lysate and not on SHySy5 cells. You may need to test different dilutions 1/200, 1/100 and/or load more protein.

Unfortunately, It is not clear how much protein was loaded onto the gel. Have you checked the detection system? Is the secondary antibody compatible with the primary antibody?

If you wish to proceed with this enquiry, it would be appreciated to complete the attached Questionnaire.

I am particularly interested in the following information:

- Sample preparation and lysis buffer;

- Total cell lysate or membrane fractions used,

- Loaded amount of protein;

- Blocking conditions

- Host species and specificity of the secondary antibody used,

- Positive control i.e human total brain lysate,

- Order Number and Your PON (order confirmation),

- Date of arrival

I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

Read More

Question

Lot number: GR 104155-1
Inquiry: please send me the detailed Secondary antibody conditions (diluent/dilution, incubation time/temperature) I tried the recommended primary antibody dilution I microgram per ml(1 hr to overnight incubation) and secondary 1:5000 RT for 1 hr, 2 hr...it is not working. I use SHySy5 cell line...and this protein is expressed in large amount as per litreature and our previous results..

Read More
Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Read More

Question

I am doing ICC and IHC with your anti-5-HT1BR antibody (https://www.abcam.com/5HT1B-Receptor-antibody-ab13896.html) and I was wondering if I could purchase the synthetic peptides used as immunogens for this antibody.

Read More
Answer

Thank you for contacting us.
The immunogens are not commercially available at this time.
Please let us know if you have any other questions.

Read More

Question

Thank you for the response.
No, we have not tried a negative control and no, we have not yet tried any other antibodies on these same extracts. Other extracts using the same extraction protocol have produced good results with western blotting however.

Read More
Answer

Thanks for your reply.

It is good to know that you have used the Trizol extraction method successfully with other samples. The reason I suggested the negative control sample was because it would, if there is an issue with the antibody specificity here, it would be confirmed with the presence of many non-specific bands in a sample that does not express 5HT1B. However if a blot against this negative control sample is blank or has one of the intense bands is missing as compared to the hamster brain samples, that could confirm there is specific detection of 5HT1B and perhaps further optimization may then be worthwhile.

What do you think?

Thanks for your troubleshooting efforts!

Read More

Question


Inquiry: I am experiencing many bands on my western blot using this antibody. Samples were extracted from hamster brain tissue using TRIzol following manufacturer instructions. I diluted my protein samples 1:1 in Laemmli buffer with beta-mercaptoethanol and boiled 5 min. Then loaded 10 ul of each sample into the gel. I ran the gel at 200V for 30min with 1X running buffer (Tris base, glycine, SDS in water). Then I transferred the gel in 1X transfer buffer (methanol, Tris base, glycine in water). Membrane was blocked 1 h in 4% dry milk. Primary antibody (1:750 in TBST) was incubated overnight at 4C. Membrane was washed 3x 5 min each in TBST, then secondary antibody was applied (1:5000 in TBST) for 1 h at room temp. Washed again in TBST, applied 1:1 Luminol/enhancer and peroxide buffer solution 3-5 min, exposed to xray film 30sec and developed in the dark. There appears to be a clear band around the expected size of 47 kDa. I ran a mouse brain sample as a control. Hamster samples seem to have the same band at 47kDa. However there are many other strong bands in both hamster and mouse samples. Both larger and smaller than band of interest. I've attached a picture. Samples are numbered, right-most column is mouse sample for comparison.

Read More
Answer

Thanks again for submitting your protocol details as well as the western blotting image.

I just have a couple additional questions:

1) Have you run a negative control sample that you expect would not contain 5HT1B?

2) Have you used these same brain extracts in western blots with any other antibodies?

Thanks for the additional information!

Read More

Question

Inquiry: I am experiencing many bands on my western blot using this antibody. Samples were extracted from hamster brain tissue using TRIzol following manufacturer instructions. I diluted my protein samples 1:1 in Laemmli buffer with beta-mercaptoethanol and boiled 5 min. Then loaded 10 ul of each sample into the gel. I ran the gel at 200V for 30min with 1X running buffer (Tris base, glycine, SDS in water). Then I transferred the gel in 1X transfer buffer (methanol, Tris base, glycine in water). Membrane was blocked 1 h in 4% dry milk. Primary antibody (1:750 in TBST) was incubated overnight at 4C. Membrane was washed 3x 5 min each in TBST, then secondary antibody was applied (1:5000 in TBST) for 1 h at room temp. Washed again in TBST, applied 1:1 Luminol/enhancer and peroxide buffer solution 3-5 min, exposed to xray film 30sec and developed in the dark. There appears to be a clear band around the expected size of 47 kDa. I ran a mouse brain sample as a control. Hamster samples seem to have the same band at 47kDa. However there are many other strong bands in both hamster and mouse samples. Both larger and smaller than band of interest. I've attached a picture. Samples are numbered, right-most column is mouse sample for comparison.

Read More
Answer

Thank you for your enquiry. I am sorry to hear this vial of ab13896 is giving you trouble. I am currently reviewing the information you have generously provided and will follow up with you as soon as possible.

Thanks for your patience!

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com