Anti-58K Golgi protein 抗体 [EPR7909] - BSA and Azide free (ab248255)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7909] to 58K Golgi protein - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-58K Golgi protein antibody [EPR7909] - BSA and Azide free
58K Golgi protein 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7909] to 58K Golgi protein - BSA and Azide free -
由来種
Rabbit -
特異性
This antibody is not suitable for testing cell line samples in western blot.
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アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248255 is the carrier-free version of ab129005.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7909 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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機能
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi. -
パスウェイ
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion. -
関連疾患
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. -
配列類似性
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole. - Information by UniProt
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参照データベース
- Entrez Gene: 10841 Human
- Omim: 606806 Human
- SwissProt: O95954 Human
- Unigene: 415846 Human
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別名
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
画像
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All lanes : Anti-58K Golgi protein antibody [EPR7909] (ab129005) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 20 secondsBlocking buffer and dilution buffer concentration: 5% NFDM/TBST.
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Anti-58K Golgi protein antibody [EPR7909] (ab129005) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaThis data was developed using ab129005, the same antibody clone in a different buffer formulation.
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This data was developed using ab129005, the same antibody clone in a different buffer formulation.ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab129005, the same antibody clone in a different buffer formulation.ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human liver tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248255 は論文での使用が確認できていません。