Anti-58K Golgi protein 抗体 [58K-9] - Golgi Marker (ab27043)
Key features and details
- Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
- Suitable for: ICC, WB, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-58K Golgi protein antibody [58K-9] - Golgi Marker
58K Golgi protein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker -
由来種
Mouse -
アプリケーション
適用あり: ICC, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Hamster, Cow, Dog, Pig, Monkey, African green monkey -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Rat Liver and HeLa cells. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
58K-9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27043の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC | (3) |
Use a concentration of 5 - 10 µg/ml.
Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details. |
WB | (2) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
|
Flow Cyt (Intra) |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC
Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
Flow Cyt (Intra)
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi. -
パスウェイ
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion. -
関連疾患
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. -
配列類似性
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole. - Information by UniProt
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参照データベース
- Entrez Gene: 10841 Human
- Entrez Gene: 397517 Pig
- Entrez Gene: 89833 Rat
- Omim: 606806 Human
- SwissProt: O95954 Human
- SwissProt: P53603 Pig
- SwissProt: O88618 Rat
- Unigene: 415846 Human
see all -
別名
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
画像
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Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were PFA-fixed and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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ab27043 staining 58K Golgi protein - Golgi Marker in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab27043 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).
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All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 3 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (60)
ab27043 は 60 報の論文で使用されています。
- Zhang X et al. Exostosin glycosyltransferase 1 reduces porcine reproductive and respiratory syndrome virus infection through proteasomal degradation of nsp3 and nsp5. J Biol Chem 298:101548 (2022). PubMed: 34971707
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Yang B et al. Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly. J Virol 95:N/A (2021). PubMed: 33504601
- Jo DS et al. Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy. Autophagy 16:1989-2003 (2020). PubMed: 31964216
- Venit T et al. Transcriptional Profiling Reveals Ribosome Biogenesis, Microtubule Dynamics and Expression of Specific lncRNAs to be Part of a Common Response to Cell-Penetrating Peptides. Biomolecules 10:N/A (2020). PubMed: 33213097