Application
ChIP
Sample
Mouse Cell lysate - nuclear (Mouse ES cells)
Specification
Mouse ES cells
Type
Native ChIP (N-ChIP)
Detection step
Real-time PCR
Positive control
Taf5
Negative control
Magoh2
Other product details
Concentration
0.5 µg/µg chromatin
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Glycerol IP buffer
Additional data
Additional Notes
We used 2ug purified and sonicated genomic DNA and 4ug antibody per IP.
Detection by QPCR.
Figure shows enrichment over IgG control
Detection by QPCR.
Figure shows enrichment over IgG control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 06 2012