Key features and details
- Goat polyclonal to 4E-T
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab6034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa.
A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257.
機能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
- Information by UniProt
- 2610509L04Rik antibody
- 4E T antibody
- 4E-T antibody
ab6034 at 0.25µg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab6034 は 3 報の論文で使用されています。
- Kamenska A et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res 44:6318-34 (2016). PubMed: 27342281
- Waghray S et al. Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA 21:1335-45 (2015). PubMed: 26015597
- Marnef A et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 23:213-24 (2012). ICC/IF ; Human . PubMed: 22090346