450 nm Stop Solution for TMB Substrate (ab171529)

Submit a review Q&A (2)References (3)

製品の概要

  • 製品名

    450 nm Stop Solution for TMB Substrate
    ELISA stop solution ihc 試薬 製品一覧

  • アプリケーション

    適用あり: ELISAmore details

  • 特記事項

    Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

    Storage Instructions:

    ab171529 should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Store at +4°C. It is important to note that this product is reported to be light sensitive. Please see notes section.

  • バッファー

    pH: 1
    Constituent: 5% Methanesulphonic acid
  • Concentration information loading...

アプリケーション

Our Abpromise guarantee covers the use of ab171529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab171529 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab171529 は 3 報の論文で使用されています。

  • Martinez Viedma MDP  et al. Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses. F1000Res 8:1875 (2019). PubMed: 32201571
  • Shen Z  et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol 186:1874-1889 (2016). PubMed: 27171900
  • Hersey JS  et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). PubMed: 26551162

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Question

How long is the reacted product stable following the use of ab171529?

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Answer

At least one hour.

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Question

Why are 450 nm Stop Solution for TMB Substrates recommended for stopping TMB substrates?

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Answer

They have greater signal stability at the high end of the standard curve, which increases dynamic range and reproducibility.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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