Anti-3-Nitrotyrosine 抗体 [7A12AF6] (ab110282)
Key features and details
- Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine
- Suitable for: ICC/IF, Flow Cyt, WB, IP, In-Cell ELISA
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-3-Nitrotyrosine antibody [7A12AF6]
3-Nitrotyrosine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine -
由来種
Mouse -
特異性
ab110282 was developed to recognize only protein-bound nitrotyrosine and so is a sensitive tool for measuring protein-specific modifications from oxidative stress. -
アプリケーション
適用あり: ICC/IF, Flow Cyt, WB, IP, In-Cell ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: HL-60 cells treated with 2 mM peroxynitrite ICC/IF: HeLa cells and Human fibroblast cells treated with 1 mM peroxynitrite WB: nitrated Bovine heart mitochondria; nitrated tyrosine
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
7A12AF6 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 µg/ml.
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|
IP |
Use at an assay dependent concentration.
|
|
In-Cell ELISA |
Use a concentration of 4 µg/ml.
(0.4 µg/well). |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. |
IP
Use at an assay dependent concentration. |
In-Cell ELISA
Use a concentration of 4 µg/ml. (0.4 µg/well). |
ターゲット情報
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関連性
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. -
別名
- 3-Nitrotyrosine antibody
- 3-NT antibody
- Nitrotyrosine antibody
画像
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Immunocytochemistry image of ab110282 stained Human HeLa cells (A) and fibroblast cells (B, C).
Cells grown on slides were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Slides were treated with/without 1 mM peroxynitrite to modify exposed tyrosines to 3-nitrotyrosine. Slides were blocked and incubated with tab110282 at 2 µg/ml overnight at 4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. For reference, the mitochondria (red) were identified by HSP60 / Alexa Fluor® 594 and DAPI was used to stain the cell nuclei (blue).
HeLa cells (A) and fibroblast cells (B) show surface modification of tyrosine to 3-nitrotyrosine after exposure to peroxynitrite. While (C) unexposed fibroblast cells show no modification. -
HL-60 cells were stained with 1 µg/ml ab110282 following treatment with 2 mM peroxynitrite (blue) or vehicle control (red). No primary antibody control is shown in black. Peroxynitrite modifies tyrosine residues to 3-nitrotyrosine.
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All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/ml
Lane 1 : Bovine heart mitochondria
Lane 2 : Bovine heart mitochondria - nitrated
Lane 3 : BSA
Lane 4 : BSA - nitrated -
All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/ml
Lane 1 : Bovine heart mitochondria
Lane 2 : Bovine heart mitochondria - nitrated
Lane 3 : BSA
Lane 4 : BSA - nitrated
Prior to runnning the samples, the membrane was treated with sodium dithionite to reduce nitrotyrosine to aminotyrosine. -
All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/ml
Lane 1 : Bovine heart mitochondria
Lane 2 : Bovine heart mitochondria - nitrated
Lane 3 : BSA
Lane 4 : BSA - nitrated
In this experiment, the antibody was first blocked with free nitrotyrosine before being used to blot the membrane.
The figure shows that ab110282's binding capacity was not inhibited by the free nitrotyrosine, and so only binds to the protein-bound form.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab110282 は 26 報の論文で使用されています。
- Peugnet V et al. Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied Cardiomyocytes. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453408
- Korovila I et al. Reduced Liver Autophagy in High-Fat Diet Induced Liver Steatosis in New Zealand Obese Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 33804819
- Wardelmann K et al. Central Acting Hsp10 Regulates Mitochondrial Function, Fatty Acid Metabolism, and Insulin Sensitivity in the Hypothalamus. Antioxidants (Basel) 10:N/A (2021). PubMed: 33946318
- Ott C et al. Decreased proteasomal cleavage at nitrotyrosine sites in proteins and peptides. Redox Biol 46:102106 (2021). PubMed: 34455147
- Brassington K et al. Ebselen reduces cigarette smoke-induced endothelial dysfunction in mice. Br J Pharmacol 178:1805-1818 (2021). PubMed: 33523477