製品の概要

  • 製品名

    293T whole cell lysate
  • 特記事項

    Description: Control cell lysates from 293T human kidney cells were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 µg of cellular protein was mixed with the same volume of 2X SDS-PAGE loading buffer.

    Growth Properties: adeherent Morphology: epithelial Organ: kidney Antigen Expression: SV40 T antigen Age: fetus


    The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.

製品の特性

  • 製品の状態

    Lyophilised:Simply reconstitute to desired concentration with sterile, distilled water or 1XPBS, adding glycerol to a final concentration of 10%. Add 100 µl distilled water containing 10% glycerol to the sample for a final concentration of 2 mg/ml lysate. Heat reconstituted cell/tissue lysate at 100C for 10min, cool down and load on to gel.
  • 保存方法

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー

    pH: 6.8
    Constituents: 0.788% Tris HCl, 1.54% DTT, 2% SDS, 10% Glycerol, 0.1% Bromophenol blue
  • Concentration information loading...
  • ライゼート 備考

    The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.

参考文献

This product has been referenced in:

  • Gonzalez-Vicente A  et al. FRUCTOSE REABSORPTION BY RAT PROXIMAL TUBULES: ROLE OF SODIUM-LINKED COTRANSPORTERS AND THE EFFECT OF DIETARY FRUCTOSE. Am J Physiol Renal Physiol N/A:N/A (2018). Read more (PubMed: 30565998) »
See 1 Publication for this product

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