製品の概要

  • 製品名
    24(S)-Hydroxycholesterol ELISA Kit
    Cholesterol キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Buffer 1 24 28.2ng/ml = 9.3%
    Buffer 2 24 9ng/ml = 5.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Buffer 1 10 28.6ng/ml = 18.4%
    Buffer 2 10 11.1ng/ml = 18.5%
  • サンプルの種類
    Tissue Culture Media, Tissue Homogenate, Cerebral Spinal Fluid
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.78 ng/ml
  • 検出範囲
    0.39 ng/ml - 100 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Tissue Culture Media = 101.7 % - %
    Tissue Homogenate = 98.6 % - %
    Cerebral Spinal Fluid = 99 % - %

  • 全工程の試験時間
    2h 00m
  • ステップ
    Multiple steps standard assay
  • 製品の概要

    Abcam’s 24(S)-Hydroxycholesterol (24-OHC) kit (ab204530) is an in-vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of 24-OHC levels in tissue culture media, cerebral spinal fluid and tissue homogenate samples.


    The homeostasis and trafficking of cholesterol is an essential component of both the central and peripheral nervous system in the maintenance of neuronal tissues. Disturbances in this homeostasis may be due to the onset of various neurological diseases such as Alzheimer’s Disease, Huntington’s Disease and multiple sclerosis.


    Apolipoprotein E and Cyp46 (also known as 24S-Cholesterol Hydroxylase) are both important in the homeostasis of cerebral cholesterol  6 and thus are of clinical interest in understanding the relation of these molecules with the pathogenesis of these, and potentially other, neurodegenerative diseases.

  • 特記事項

    24-OHC, an enzymatically-generated side chain-hydroxylated derivative of cholesterol, is a pivotal marker in the study of cerebral cholesterol homeostasis. Cholesterol is unable to cross the blood-brain barrier however, Cyp46 enzyme converts cholesterol to the more soluble 24-OHC, and this hydroxylated form of cholesterol is able to cross the bloodbrain barrier.7,8 This conversion allows for the reduction of cholesterol in the brain and the efflux of 24-OHC from the brain into cerebral spinal fluid and blood. The flux of 24-OHC has been observed in patients with a variety of neurodegenerative diseases. In the instance of Alzheimer’s disease, the change in 24S-hydroxycholesterol concentrations may be indicative of different pathogenetic mechanisms and/or the progression of the disease.3 As in the case of multiple sclerosis, concentrations of 24-OHC have been shown to decrease, likely due to the loss of neuronal cells responsible for the synthesis.

  • アプリケーション
    適用あり: ELISAmore details
  • 試験プラットフォーム
    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab204530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

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プロトコール

参考文献

ab204530 has not yet been referenced specifically in any publications.

レビューと Q&A

did not work with serum/plasma

Poor Good 4/5 (Ease of Use)
Abreviews
This kit did not work with serum/plasma. Both with or without detection Ab with EDTA plasma showed the same high background levels.

Abcam user community

Verified customer

投稿 Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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