Anti-14-3-3 Theta + Tau 抗体 [EPR5123] (ab124909)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5123] to 14-3-3 Theta + Tau
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-14-3-3 Theta + Tau antibody [EPR5123]
14-3-3 Theta + Tau 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5123] to 14-3-3 Theta + Tau -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human 14-3-3 Theta + Tau (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa, 293T, A549 and A431 cell lysates; A549 cells, WB: 14-3-3 theta (YWHAQ) Human Recombinant Protein
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 6.09 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5123 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124909の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
特記事項 |
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Flow Cyt (Intra)
1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
組織特異性
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
Ser-232 is probably phosphorylated by CK1. -
細胞内局在
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
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参照データベース
- Entrez Gene: 10971 Human
- Entrez Gene: 100503129 Mouse
- Entrez Gene: 22630 Mouse
- Entrez Gene: 25577 Rat
- Omim: 609009 Human
- SwissProt: P27348 Human
- SwissProt: P68254 Mouse
- SwissProt: P68255 Rat
see all -
別名
- 14-3-3 protein T cell antibody
- 14-3-3 protein T-cell antibody
- 14-3-3 protein tau antibody
see all
画像
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All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : A549 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Intracellular flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
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All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/1000 dilution
Lane 1 : 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 2 : 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 3 : 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 4 : 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 5 : 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 6 : 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 7 : 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 secondsBlocking and dilution buffer: 5% NFDM/TBST
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124909 は 1 報の論文で使用されています。
- Gao K et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB ; Human . PubMed: 25588844