12(S)-HETE ELISA Kit (ab133034)
Key features and details
- Sensitivity: 146.3 pg/ml
- Range: 195 pg/ml - 50000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Competitive
製品の概要
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製品名
12(S)-HETE ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Buffer 16 342pg/ml = 5.2% Buffer 16 1153pg/ml = 10.1% Buffer 16 4762pg/ml = 15.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Buffer 224pg/ml = 4.1% Buffer 1127pg/ml = 9.1% Buffer 5294pg/ml = 20.8% -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Competitive -
検出感度
= 146.3 pg/ml -
検出範囲
195 pg/ml - 50000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture media = 94 % - % Hep Plasma = 104 % - % EDTA Plasma = 97 % - % -
ステップ
Multiple steps standard assay -
製品の概要
Abcam’s 12(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 12(S)-HETE in cell culture supernatants and plasma (heparin, EDTA).
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-12(S)-HETE antigen and a polyclonal rabbit antibody specific to 12(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 12(S)-HETE captured in the plate.
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特記事項
12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.
Cross Reactivity
Compound % Cross Reactivity 12(S)-HETE 100 12(R)-HETE 2.5 15-HETE 0.3 5(S)-HETE 0.2 8,15-diHETE 0.1 5,15-diHETE 0.1 PGE2 0.1 PGF2α 0.1 PGD2 0.1 6-keto-PGF1α 0.1 Thromboxane B2 0.1 Arachidonic Acid 0.1 Leukotriene B4 0.1 Leukotriene C4 0.1 Leukotriene D4 0.1 Leukotriene E4 0.1 8-HETE <0.1
9-HETE <0.1 11-HETE <0.1 -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 12(S)-HETE Antibody 1 x 5ml 12(S)-HETE Conjugate 1 x 5ml 12(S)-HETE Standard 1 x 0.5ml 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究分野
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関連性
12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab133034 は 7 報の論文で使用されています。
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- Bao Z et al. MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma. Neuro Oncol 23:2014-2027 (2021). PubMed: 33984142
- Bao Z et al. Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury. Nat Commun 12:4220 (2021). PubMed: 34244497
- Du Y et al. Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells. Oncol Lett 22:781 (2021). PubMed: 34594422
- Cheng J et al. ACSL4 suppresses glioma cells proliferation via activating ferroptosis. Oncol Rep 43:147-158 (2020). PubMed: 31789401
- Tan S et al. Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis. Oncol Lett 20:122 (2020). PubMed: 32863935
- Li Y et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion. Cell Death Differ N/A:N/A (2019). PubMed: 30737476