Anti-12 Lipoxygenase/ALOX12 抗体 - C-terminal (ab211506)
Key features and details
- Rabbit polyclonal to 12 Lipoxygenase/ALOX12 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal
12 Lipoxygenase/ALOX12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 12 Lipoxygenase/ALOX12 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human 12 Lipoxygenase/ALOX12 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. NCBI Accession No. NP_000688.2.
Database link: P18054 -
ポジティブ・コントロール
- WB: K562 cell lysate. IHC-P: Human heart tissue. ICC/IF: A549 cells. Flow Cyt (Intra): K562 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab211506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/50.
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WB |
1/1000. Predicted molecular weight: 76 kDa.
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ICC/IF |
1/10 - 1/50.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/50. |
WB
1/1000. Predicted molecular weight: 76 kDa. |
ICC/IF
1/10 - 1/50. |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Oxygenase and 14,15-leukotriene A4 synthase activity. -
パスウェイ
Lipid metabolism; leukotriene D4 biosynthesis. -
配列類似性
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 239 Human
- Omim: 152391 Human
- SwissProt: P18054 Human
- Unigene: 654431 Human
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別名
- 12 LOX antibody
- 12(S) lipoxygenase antibody
- 12-lipoxygenase antibody
see all
画像
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Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506) at 1/1000 dilution + K562 cell lysate at 35 µg
Predicted band size: 76 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/50 dilution.
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Immunofluorescence analysis of A549 cells, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1/400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
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Intracellular Flow Cytometry analysis of K562 cells labeling 12 Lipoxygenase/ALOX12 (bottom histogram), using ab211506 at a 1/10 dilution, and negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab211506 は 8 報の論文で使用されています。
- Yang J et al. Development and validation of a novel 3-gene prognostic model for pancreatic adenocarcinoma based on ferroptosis-related genes. Cancer Cell Int 22:21 (2022). PubMed: 35033072
- Kan X et al. Newcastle-disease-virus-induced ferroptosis through nutrient deprivation and ferritinophagy in tumor cells. iScience 24:102837 (2021). PubMed: 34368653
- Tomita K et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci 22:N/A (2021). PubMed: 34361070
- Chen Y et al. Baicalein Alleviates Erectile Dysfunction Associated With Streptozotocin-Induced Type I Diabetes by Ameliorating Endothelial Nitric Oxide Synthase Dysfunction, Inhibiting Oxidative Stress and Fibrosis. J Sex Med 17:1434-1447 (2020). PubMed: 32586748
- Tacconelli S et al. Pharmacological characterization of the biosynthesis of prostanoids and hydroxyeicosatetraenoic acids in human whole blood and platelets by targeted chiral lipidomics analysis. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158804 (2020). PubMed: 32853794
- Dakin SG et al. 15-Epi-LXA4 and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture. FASEB J N/A:fj201900196R (2019). PubMed: 30916999
- Tomita K et al. Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA. Cancer Sci 110:2856-2866 (2019). PubMed: 31314163
- Dakin SG et al. Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears. Am J Pathol 189:2258-2268 (2019). PubMed: 31437425