10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)
製品の概要
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製品名10x Heat Mediated Antigen Retrieval Solution pH 6.0
Antigen retrieval solution ihc 試薬 製品一覧 -
アプリケーション適用あり: IHC-Pmore details
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特記事項This Antigen retrieval solution is citrate buffer pH6. It incorporates ASSURE™, which is a color-coded pH indicator technology. The end-user is assured, by visual inspection, that the solution is at the correct dilution and pH. The 10x solution is orange. The 1X solution is yellow. When boiling, the solution becomes a pale orange. Should the pH rise above 7.0, the solution will turn a reddish color.
This Antigen retrieval solution is a 10x buffer solution with paraffin emulsifier, allowing the user to deparaffinize and pretreat tissues in one-step. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. This product is specially formulated for superior pH stability at high temperature, and will help prevent the possibility of losing pH sensitive antigens.
製品の特性
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製品の状態Liquid
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保存方法Store at room temperature.
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バッファーpH: 7.30
Preservative: Proprietary preservative
Constituents: PBS, Carrier protein
Note: 10x concentrated. -
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IHC 試薬 備考This Antigen retrieval solution is a 10x buffer solution with paraffin emulsifier, allowing the user to deparaffinize and pretreat tissues in one-step. Xylenes and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. This product is specially formulated for superior pH stability at high temperature, and will help prevent the possibility of losing pH sensitive antigens.
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. Please see the protocol page for more details. |
参考文献
This product has been referenced in:
- Wu M et al. Ga13 negatively controls osteoclastogenesis through inhibition of the Akt-GSK3ß-NFATc1 signalling pathway. Nat Commun 8:13700 (2017). Read more (PubMed: 28102206) »
- Benavides OM et al. Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly(ethylene glycol) hydrogels. Tissue Eng Part A 21:1185-94 (2015). IHC-P . Read more (PubMed: 25517426) »
