10x EDTA Buffer pH 8.0 (ab64216)
製品の概要
-
製品名
10x EDTA Buffer pH 8.0 -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).
Recommended Procedure: Microwave
- Deparaffinize and rehydrate tissue sections.
- Dilute the product 1:10 with deionized water.
- Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
- Keep the slides warm by heating for 10min at LOW power.
- Let coplin jar sit in microwave for at least 20min.
- Remove and rinse with buffer.
- Proceed with staining.
製品の特性
-
製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 7.7
Constituent: 0.29% EDTA
Buffer 10x concentrated. -
Concentration information loading...
-
研究分野
-
関連性
EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies. -
別名
- Ethylenediaminetetraacetic acid buffer
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64216の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent dilution.
|
特記事項 |
---|
IHC-P
Use at an assay dependent dilution. |
画像
-
ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab64216 は 2 報の論文で使用されています。
- Posta N et al. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction. Lab Invest 100:986-1002 (2020). PubMed: 32054994
- Hoang JD et al. Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y. JCI Insight 5:N/A (2020). PubMed: 32493842