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  1. Link

    10x-annexin-vanxa5-binding-buffer-ab14084.pdf

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Cell Biology Apoptosis Annexin V Reagents
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10X Annexin V/ANXA5 Binding Buffer (ab14084)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (2)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

こちらの製品もご検討ください

アッセイ
Product image
Propidium Iodide (ab14083)
Biochemical
Product image
Calcein AM, fluorescent dye for cell viability (ab141420)
ELISA
Product image
Human Annexin V ELISA Kit (ab223863)

関連製品

製品の概要

  • 製品名

    10X Annexin V/ANXA5 Binding Buffer
    Annexin V/ANXA5 ihc 試薬 製品一覧
  • アプリケーション

    適用あり: Flow Cyt, FMmore details
  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called 1006 10X Annexin V Binding Buffer. 1006-100 is the same size as the 100 ml size of ab14084.

    The binding buffer contains optimal concentration of calcium which is required for Annexin v/ANXA5 binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.

    This buffer can be used in conjunction with our Annexin V/ANXA5 reagents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Store at +4°C.
  • バッファー

    pH: 7.40

    Concentrated x10
  • Concentration information loading...
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents
  • 機能

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 関連疾患

    Pregnancy loss, recurrent, 3
  • 配列類似性

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • ドメイン

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻訳後修飾

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 別名

    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all

関連製品

  • Related Products

    • Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
    • Propidium Iodide (ab14083)
    • Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
    • Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
    • Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
    • Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
    • Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
    • Annexin V-EGFP Apoptosis Staining / Detection Reagent (ab14152)
    • Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
    • Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
    • Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
    • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
    • Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14084の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
FM
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.
FM
Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (2)

ab14084 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14084 は 2 報の論文で使用されています。

  • Paez-Mayorga J  et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 5:125 (2018). PubMed: 30238007
  • Grant R  et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). PubMed: 21741384

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Question

Dear All,
a customer of ours bought ab14085 (Annexin V apoptosis detection kit), but he needs to buy Annexin V-Fitc and Annexin V binding buffer separately.
Can you please confirm that the following codes are the same included within the kit ab14085:


Annexin V-Fitc #ab14082

Annexin V binding buffer #ab14084

Thanks in advance and kind regards

Read More

Abcam community

Verified customer

Asked on Sep 17 2012

Answer

Thank you for your inquiry.

I can confirm that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are the same reagents as in the kit ab14085(Annexin V-FITC Apoptosis Detection Kit).

Please note that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are more concentrated and need need to be diluted to 1x before using them.

I hope this information is helpful and wish you and your customer a good week.

Read More

Abcam Scientific Support

Answered on Sep 17 2012

Question

Thanks for information. I do not have time to look with whom I should
contact, but I recently ordered ab14084 Annexin-V binding buffer and it
does not work at all, whereas the equal buffers from BD Sciences and
Beckman Coulter worked fine. Your FITC labelled Annexoin V is fine.
Any suggestions?
Best,

Read More

Abcam community

Verified customer

Asked on Mar 08 2012

Answer

Thank you for taking the time to contact us. Your enquiry has been forwarded to the Scientific Support tem. I am sorry to hear you have had difficulty obtaining satisfactory results when using this buffer.

I would like to reassure you that this buffer is covered by our 6 month guarantee. In the event that a product is not functioning as stated on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would aprpeciate if you could provide the following information.

1. What was the order number and date of purchase?

2. Please provide a description of the experiment this buffer has been used in.

3. What type of samples were used?

4. I would appreciate if you could also provide any images/data which would help us to assess the results.

Thank you for your time and cooperation.I look forward to hearing from you with the requested inoformation and details of how you would like to proceed.

Read More

Abcam Scientific Support

Answered on Mar 08 2012

Question

Dear Techserve This is the customers wish: Then I would prefer lot GR63048-1.   Thanking you -  

Read More

Abcam community

Verified customer

Asked on Nov 07 2011

Answer

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab14084 as a free of charge replacement. I hope the second bottle will work as it is expected, and please do let me know how you are getting on with this product.  

Read More

Abcam Scientific Support

Answered on Nov 07 2011

Question

Please make sure the replacement bottle is from lot#GR48964-2.     Thanks a lot for dealing with this enquiry. Best Regards,  

Read More

Abcam community

Verified customer

Asked on Nov 04 2011

Answer

Thank you for your response. I can certainly place a new order for your customer, however regrettably we do no longer have the requested batch: GR48964-2. It has all been sold out. Currently we have  GR59518-1 and GR63048-1 in our stock. Please do let me know how you wish to proceed.  I look forward to hearing from you soon.

Read More

Abcam Scientific Support

Answered on Nov 04 2011

Question

 We just received this complaint from the customer below:  I ordered Oct, 12 (order 3-4901676515) 2x 50ml Abcam Annexin V 10x Binding Buffer ab14084. I received 2 bottles coming from 2 different lots. Doing my assays end last week the Annexin staining didn’t work. I investigated the 2 lots and saw that lot #GR59518-1 which was used doesn’t work whereas lot#GR48964-2 works perfectly. I have 20ml left from #GR59518-1. What shall I do, sending it back for an exchange and to where? It’s really annoying when a big sophisticated experiment is failing do to a faulty buffer.  Thank you for your quick reply in how you handling this matter! Yours kindly

Read More

Abcam community

Verified customer

Asked on Nov 03 2011

Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Although the batches are different, both bottles are from the same internal lot number. I regret to inform you that this means that there should be no difference between the two bottles. Just to clarify, the internal lot number is an identification number for the synthesis/purification/manufacturing process. The product can be placed to our different stocks in different times and the batch numbers (GR48964-2 and GR59518-1) represent when the actual aliquots were placed into our stock. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.  I can certainly offer a new free of charge replacement bottle if your customer would like to get one. Please do let me know how your customer would like to proceed. I look forward to hearing from you soon.  

Read More

Abcam Scientific Support

Answered on Nov 03 2011

Question

Customer Question: I was wondering what the AnnexinV Binding Buffer is made of.

Read More

Abcam community

Verified customer

Asked on May 04 2005

Answer

Thank you for your enquiry. The buffer is made of Tris-based physiological buffer containing the optimal calcium concentration. Please contact us again if you require further information.

Read More

Abcam Scientific Support

Answered on May 04 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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