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PPQPREKVSSIDLEIDSconjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 134-150 of Human Zyxin
Our Abpromise guarantee covers the use of ab50391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 61 kDa.|
|ELISA||Use at an assay dependent dilution.
Array: Use at an assay dependent dilution.
|ICC||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
ab50391 staining rat neuronal progenitor cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 4 µg/ml. An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.