製品の概要

  • 製品名Anti-Zyxin antibody [EPR4302]
    Zyxin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4302] to Zyxin
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Zyxin.

  • ポジティブ・コントロール
    • WB: HeLa, MCF7 and C2C12 cell lysates IHC-P: Human kidney tissue ICC/IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 関連性Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • 細胞内局在Cytoplasmic, cytoskeletal and Nuclear.
  • 参照データベース
  • 別名
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Anti-Zyxin antibody [EPR4302] 画像

  • Overlay histogram showing HeLa cells stained with ab109316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109316, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-Zyxin antibody [EPR4302] (ab109316) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : MCF7 lysate
    Lane 3 : C2C12 lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Zyxin in paraffin embedded Human kidney tissue, using ab109316 at a 1/250 dilution.
  • Immunofluorescent staining of Zyxin in HeLa cells, using ab109316 at a 1/100 dilution.

Anti-Zyxin antibody [EPR4302] (ab109316) 使用論文

This product has been referenced in:
  • Qi L  et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci 129:3661-3674 (2016). Read more (PubMed: 27694340) »
  • Goodwin JM  et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). Read more (PubMed: 25042806) »

See all 3 Publications for this product

Product Wall



For IHC, we used a MACH 2 Rabbit HRP-Polymer secondary and for ICC, we used an anti-rabbit Alexa Fluor 568.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"