製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 関連性Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • 細胞内局在Cytoplasmic, cytoskeletal and Nuclear.
  • 参照データベース
  • 別名
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Anti-Zyxin antibody 画像

  • All lanes : Anti-Zyxin antibody (ab71842) at 1 µg/ml

    Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 5 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 6 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • Zyxin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Zyxin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71842.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 75kDa; Zyxin
  • ICC/IF image of ab71842 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) Hela, Hek293, HepG2, and MCF-7 cells at 5µg/ml.

Anti-Zyxin antibody (ab71842) 使用論文

This product has been referenced in:
  • Van Itallie CM  et al. Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci 127:885-95 (2014). Read more (PubMed: 24338363) »
  • Ketscher A  et al. LSD1 controls metastasis of androgen-independent prostate cancer cells through PXN and LPAR6. Oncogenesis 3:e120 (2014). WB, IP ; Human . Read more (PubMed: 25285406) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (7% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 24 2013

DISCOUNT CODE: xxx for ab19347
DISCOUNT CODE: xxx for ab71842
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I am very pleased to hear you would like to accept our offer and test ab19347 and ab71842in chicken and/or IHC-Fr. Each code will give you: 1 free P...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton x-100 in PBS
Blocking step Milk as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Mrs. Merly Saare

Verified customer

投稿 Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount 100 µg
Specification HeLa, Jurkat
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Mrs. Merly Saare

Verified customer

投稿 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"