Rabbit polyclonal to ZWINT
Synthetic peptide corresponding to a region between residue 225 and the C terminus (residue 277) of human ZWINT (NP_008988.2).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab71982 was affinity purified using an epitope specific to ZWINT immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 - 1/250.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
Information by UniProt
Human ZW10 interacting protein 1 antibody
HZwint 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (ab71982)
Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - Anti-ZWINT antibody (ab71982)
All lanes :
Anti-ZWINT antibody (ab71982) at 0.04 µg
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
38 kDa (
why is the actual band size different from the predicted?
Additional bands at:
28 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-ZWINT antibody (ab71982)
Detection of ZWINT by Western Blot of Immunprecipitate.
ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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