製品の概要

  • 製品名Anti-ZP1 antibody
    ZP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZP1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide designed within residues: VGFEDSYGQE PTLGPTDSNG NSSLRPLLWA VLLLPAVALV LGFGVFVGLS, corresponding to internal amino acids 576 - 625 of Human ZP1 (NP_997224).

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
  • 組織特異性Oocytes.
  • 配列類似性Belongs to the ZP domain family. ZPB subfamily.
    Contains 1 P-type (trefoil) domain.
    Contains 1 ZP domain.
  • ドメインThe ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • 翻訳後修飾Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    O-glycosylated.
  • 細胞内局在Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC87693 antibody
    • Processed zona pellucida sperm-binding protein 1 antibody
    • zona pellucida glycoprotein 1 (sperm receptor) antibody
    • Zona pellucida glycoprotein 1 antibody
    • zona pellucida protein 1 antibody
    • Zp 1 antibody
    • Zp-1 antibody
    • Zp1 antibody
    • ZP1_HUMAN antibody
    see all

Anti-ZP1 antibody 画像

  • Anti-ZP1 antibody (ab90480) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa

Anti-ZP1 antibody (ab90480) 使用論文

ab90480 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting my colleague at Abcam. To our knowledge, no one has tested this for ICC/IF. Trying a different fixative, for instance methanol instead of paraformaldehyde, may give a better result, but we have not tried either.

I r...

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