製品の概要

  • 製品名
    Anti-zona radiata antibody [MN-7F2]
    zona radiata 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MN-7F2] to zona radiata
  • 特異性
    This antibody binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the g monomer.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Rainbow trout
  • 免疫原

    Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).

  • ポジティブ・コントロール
    • Plasma from 17ß-estradiol treated Salmon.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Affinity purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    MN-7F2
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/100 - 1/5000.
    WB: 1/100 - 1/1000. Detects a band of approximately 45 kDa.
    The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Zona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
    • 参照データベース
      • 別名
        • ZRP antibody

      画像

      • Coating: Plasma (diluted 1:1000) from 17ß-estradiol treated (E2) or control (C) fish.
      • Lane 1 : Molecular weight standards
        Lanes 2 - 10 : Anti-zona radiata antibody [MN-7F2] (ab36802) at 1/500 dilution

        Lane 1 : As above
        Lane 2 : Purified Zrp, [alpha, beta and gamma].
        Lane 3 : Plasma from 17ß-estradiol treated Salmon at 1/200 dilution
        Lane 4 : Plasma from control Salmon at 1/200 dilution
        Lane 5 : Plasma from 17ß-estradiol treated Arctic char at 1/200 dilution
        Lane 6 : Plasma from control Arctic char at 1/200 dilution
        Lane 7 : Plasma from 17ß-estradiol treated Turbot at 1/200 dilution
        Lane 8 : Plasma from control Turbot at 1/200 dilution
        Lane 9 : Plasma from 17ß-estradiol treated Cod at 1/200 dilution
        Lane 10 : Plasma from control Cod at 1/200 dilution

      プロトコール

      参考文献

      ab36802 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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