製品の概要

  • 製品名
    Anti-ZO1 tight junction protein antibody
    ZO1 tight junction protein 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to ZO1 tight junction protein
  • 特異性
    ab99462 is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Peptide with sequence

    C-PKPTSQNQFSEHDKT

    , from the internal region of the protein sequence according to NP_003248.3; NP_783297.2.

  • ポジティブ・コントロール
    • Human brain (cerebral cortex) lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 195 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24874215

ターゲット情報

  • 機能
    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • 組織特異性
    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • 配列類似性
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • ドメイン
    The second PDZ domain mediates interaction with GJA12.
  • 翻訳後修飾
    Phosphorylated. Dephosphorylated by PTPRJ.
  • 細胞内局在
    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody 画像

  • Anti-ZO1 tight junction protein antibody (ab99462) at 0.3 µg/ml + Human brain (cerebral cortex) lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 195 kDa


    Exposure time : 1 hour

Anti-ZO1 tight junction protein antibody (ab99462) 使用論文

This product has been referenced in:
  • Lundin A  et al. Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog 10:e1004166 (2014). ICC/IF ; Human . Read more (PubMed: 24874215) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris
Specification
Lung
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Feb 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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