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Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.
Our Abpromise guarantee covers the use of ab96587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 187 kDa.|
Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for ZO1 tight junction protein (green) using ab96587 at 1/200 dilution in ICC/IF. Blue: Hoechst 33342 staining.
Paraffin-embedded H661 xenograft tissue stained for ZO1 tight junction protein using ab96587 at 1/250 dilution in immunohistochemical analysis.
Immunohistochemical analysis of paraffin embedded mouse intestine tissue labeling ZO1 with ab96587 at 1/500 dilution.
Immunohistochemical analysis of paraffin embedded mouse testis labeling ZO1 with ab96587 at 1/500 dilution.
ab96587 staining ZO1 tight junction protein in NT2D1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/200) stained green. Nuclei stained blue with Hoechst.