Anti-ZO1 tight junction protein 抗体 (ab84934)

製品の概要

  • 製品名Anti-ZO1 tight junction protein antibody
    ZO1 tight junction protein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZO1 tight junction protein
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human ZO1 tight junction protein.

    (Peptide available as ab101198.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Lung Tissue Lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 195 kDa).

ターゲット情報

  • 機能The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • 組織特異性The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • 配列類似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • ドメインThe second PDZ domain mediates interaction with GJA12.
  • 翻訳後修飾Phosphorylated. Dephosphorylated by PTPRJ.
  • 細胞内局在Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody 画像

  • Anti-ZO1 tight junction protein antibody (ab84934) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 195 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-ZO1 tight junction protein antibody (ab84934) 使用論文

ab84934 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84934.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"