The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
機能E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to substrates. May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin-protein ligase activity.
組織特異性Expressed primarily in the nervous system, with expression higher in developing brain relative to adult. Expressed at low levels in testis and thymus.
パスウェイProtein modification; protein ubiquitination.
配列類似性Contains 1 RING-type zinc finger.
ドメインThe RING-type zinc finger domain is required for E3 ligase activity.
細胞内局在Endosome. Lysosome. Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Associated with synaptic vesicle membranes in neurons.