製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF81
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide: GKAFTNRSNL NTHQKSHTGE KSYICAECGK AFTDRSNFNK HQTIHTGEKP, corresponding to a region within internal sequence amino acids 503-553 of Human ZNF81

  • ポジティブ・コントロール
    • Jurkat cell lysats Human liver tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    May be involved in transcriptional regulation.
  • 関連疾患
    Defects in ZNF81 are the cause of mental retardation X-linked type 45 (MRX45) [MIM:300498]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Note=A chromosomal aberration involving ZNF81 is found in a severe mental retardation patient. Translocation t(X;9)(p11.23;q34.3).
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 12 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HFZ20 antibody
    • Zinc finger protein 81 antibody
    • ZNF81 antibody
    • ZNF81_HUMAN antibody

画像

  • Anti-ZNF81 antibody (ab49119) at 0.5 µg/ml + Jurkat cell lysate

    Predicted band size : 76 kDa
  • Immunohistochemical analysis staining ZNF81 in Human liver tissue sections using ab49119 at 4μg/ml.

参考文献

ab49119 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (K562 nuclear extract)
Total protein in input
25 µg
Specification
K562 nuclear extract
Immuno-precipitation step
Protein A/G
Username

Dr. Seth Frietze

Verified customer

投稿 Oct 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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