製品の概要

  • 製品名
  • 製品の詳細
    Rat polyclonal to ZNF785
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: RKTALEAHRW IHRSCSERRA WQQAVVGRSE PIPVLGGKDP PVHFRHFPDI, corresponding to amino acids 351-400 of Human ZNF785

  • ポジティブ・コントロール
    • WB: Transfected 293T cell lysate IHC-P: Human intestinal tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with ZNF785 peptide (ab113081). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

ターゲット情報

画像

  • Anti-ZNF785 antibody (ab50868) at 0.5 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling ZNF785 with ab50868 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of human intestinal villus. Magnification: 400X.

参考文献

ab50868 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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