製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF773
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within residues: AAATLRDPAQ QGYVTFEDVA VYFSQEEWRL LDDAQRLLYR NVMLENFTLL, corresponding to N terminal amino acids 2-51 of Human ZNF773 (NP_940944)

  • ポジティブ・コントロール
    • Human placenta lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    May be involved in transcriptional regulation.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ00301 antibody
    • MGC4728 antibody
    • Zinc finger protein 419B antibody
    • Zinc finger protein 773 antibody
    • ZN773_HUMAN antibody
    • ZNF419B antibody
    • ZNF773 antibody
    see all

画像

  • Anti-ZNF773 antibody (ab83103) at 1 µg/ml + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Ramiro-Diaz JM  et al. Mechanisms of NFATc3 activation by increased superoxide and reduced hydrogen peroxide in pulmonary arterial smooth muscle. Am J Physiol Cell Physiol 307:C928-38 (2014). Read more (PubMed: 25163518) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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