Anti-ZNF574 抗体 (ab85933)
Key features and details
- Rabbit polyclonal to ZNF574
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ZNF574 antibody -
製品の詳細
Rabbit polyclonal to ZNF574 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to a region between residues 846 and 896 of human ZNF574 (NP_073589.4).
-
ポジティブ・コントロール
- HeLa and 293T whole cell lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab85933 was affinity purified using an epitope specific to ZNF574 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85933の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
May be involved in transcriptional regulation. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 20 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 767618 Cow
- Entrez Gene: 64763 Human
- Entrez Gene: 100173939 Orangutan
- SwissProt: Q29RK0 Cow
- SwissProt: Q6ZN55 Human
- SwissProt: Q5R4P8 Orangutan
- Unigene: 726232 Human
-
別名
- FLJ22059 antibody
- FP972 antibody
- Zinc finger protein 574 antibody
see all
画像
-
All lanes : Anti-ZNF574 antibody (ab85933) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
developed using the ECL technique -
Detection of human ZNF54 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85933 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab85933 は論文での使用が確認できていません。