製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF543
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: GCPLFKPELI YQLDHRQELW MATKDLSQSS YPGDNTKPKT TEPTFSHLAL PEEVLLQEQL TQGASKNSQL GQSK, corresponding to amino acids in Human ZNF543.

  • ポジティブ・コントロール
    • Human rectum tissue; RT 4, U 251 MG, Human plasma, liver and tonsil lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

  • 機能
    May be involved in transcriptional regulation.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 13 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp434H055 antibody
    • Zinc finger protein 543 antibody
    • ZN543_HUMAN antibody
    • ZNF543 antibody

画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122080 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122080, at 1/50 dilution, staining ZNF543 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-ZNF543 antibody (ab122080) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

参考文献

ab122080 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab122080.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録