製品の概要

  • 製品名
    Anti-ZNF524 antibody
    ZNF524 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZNF524
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PGGATSSNRT LKASLPRKRG RPPKSGQEPP LVQVQGVTAP VGSSGGSDLL) of Human ZNF524 (NP_694951).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • MCF7 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Western blot - ZNF524 antibody (ab87224)
    Western blot - ZNF524 antibody (ab87224)
    Anti-ZNF524 antibody (ab87224) at 1 µg/ml (5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Gel concentration 12%

プロトコール

参考文献

ab87224 has not yet been referenced specifically in any publications.

ab87224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Immunoprecipitation abreview for Anti-ZNF524 antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (SILAC labeled HeLa S3 nuclear extract)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
SILAC labeled HeLa S3 nuclear extract
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投稿 Apr 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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