Anti-ZNF511 抗体 (ab87137)

製品の概要

  • 製品名
    Anti-ZNF511 antibody
  • 製品の詳細
    Rabbit polyclonal to ZNF511
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (QLPPALCARL AAGPGAAEPL PVERDPAAGA APFRFVARPV RFPREHQFFE) of Human ZNF511 (NP_665805).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-ZNF511 antibody (ab87137) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cel lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : ~35 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab87137 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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投稿 Sep 22 2014

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