製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF485
  • 特異性
    This antibody reacts with ZNF485
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: RHSSGLVEHQ RLHTGEKPYK CNECGKAFPR SSALKQHKKI HNKERAMKCS, corresponding to amino acids 392-441 of Human ZNF485

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Lane 1 :
    Lane 2 : Anti-ZNF485 antibody (ab50559) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 46 kDa
    Observed band size : 35,50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab50559 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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