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A region within synthetic peptide: LKNQTPIEER GDLFGKALNL NTDFVSLRQV PYKYDLYEKT LKYNSDLLNS, corresponding to N terminal amino acids 73-122 of Human ZNF475
Our Abpromise guarantee covers the use of ab49223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
ab49223 has not yet been referenced specifically in any publications.