製品の概要
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製品名Anti-ZNF462 antibody
ZNF462 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ZNF462
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region within amino acids 2456-2506 of Human ZNF462 (NP_067047.4).
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ポジティブ･コントロール
- 293T whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab117771 was affinity purified using an epitope specific to ZNF462 immobilized on solid support.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab117771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 285 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能May be involved in transcriptional regulation.
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配列類似性Contains 27 C2H2-type zinc fingers.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 58499 Human
- SwissProt: Q96JM2 Human
- Unigene: 370379 Human
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別名
- DKFZp686B2325 antibody
- DKFZp762N2316 antibody
- FLJ14960 antibody
see all
画像
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Western blot - Anti-ZNF462 antibody (ab117771)All lanes : Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 285 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZNF462 antibody (ab117771)Detection of ZNF462 by Western Blot of Immunprecipitate.
ab117771 at 1µg/ml staining ZNF462 in 293T whole cell lysate immunoprecipitated using ab117771 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
参考文献
This product has been referenced in:
- Devkota S et al. Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy 12:2038-2053 (2016). Read more (PubMed: 27541728) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"