Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-ZNF446 antibody 画像
Western blot - ZNF446 antibody (ab66657)
Anti-ZNF446 antibody (ab66657) at 1.25 µg/ml + (B) HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
49 kDaObserved band size :
55 kDa (why is the actual band size different from the predicted?
)Additional bands at :
36 kDa. We are unsure as to the identity of these extra bands.
Anti-ZNF446 antibody (ab66657) 使用論文
has not yet been referenced specifically in any publications.
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