製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF426
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A region within synthetic peptide: VMLENYKNLA TVGGQIIKPS LISWLEQEES RTVQGGVLQG WEMRLETQWS, corresponding to N terminal amino acids 70-119 of Human ZNF426

  • ポジティブ・コントロール
    • Fetal liver lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-ZNF426 antibody (ab30967) at 0.5 µg/ml (in 5% skim milk / PBS) + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab30967 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Multiple cell types)
Loading amount
30 µg
Specification
Multiple cell types
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Seth Frietze

Verified customer

投稿 Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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