ab54360 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 44 kDa for the fragment (predicted molecular weight for the full length protein: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcription factor that binds the consensus DNA sequence [GC]AAAAA. Seems to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1.
Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 8 C2H2-type zinc fingers.