製品の概要

  • 製品名Anti-ZNF365 antibody
    ZNF365 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZNF365
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: RFRSLSSLRA HLEFSHSYEE RTLLTKCSLF PSLKDTDLVT SSELLKPGKL, corresponding to N terminal amino acids 36-85 of Human ZNF365

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

Anti-ZNF365 antibody 画像

  • Anti-ZNF365 antibody (ab49243) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ZNF365 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

Anti-ZNF365 antibody (ab49243) 使用論文

ab49243 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"