製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF364
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide derived from a region encoded by C terminal amino acids 254-303 (IVPWLELHDT CPVCRKSLNG EDSTRQSQST EASASNRFSN DSQLHDRWTF) of Human ZNF364

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
  • 組織特異性
    Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾
    RING-type zinc finger-dependent and E2-dependent autoubiquitination.
  • 細胞内局在
    Cytoplasm > cytosol. The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCA2 antibody
    • Breast cancer associated gene 2 antibody
    • CL469780 antibody
    • E3 ubiquitin-protein ligase RNF115 antibody
    • Rabring 7 antibody
    • RING finger protein 115 antibody
    • RN115_HUMAN antibody
    • RNF115 antibody
    • Zinc finger protein 364 antibody
    see all

画像

  • Anti-ZNF364 antibody (ab80432) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

プロトコール

参考文献

This product has been referenced in:
  • Nityanandam R & Serra-Moreno R BCA2/Rabring7 targets HIV-1 Gag for lysosomal degradation in a tetherin-independent manner. PLoS Pathog 10:e1004151 (2014). WB . Read more (PubMed: 24852021) »
  • Rodrigo-Brenni MC  et al. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell 55:227-37 (2014). Human . Read more (PubMed: 24981174) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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