The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
組織特異性Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.
パスウェイProtein modification; protein ubiquitination.
配列類似性Contains 1 RING-type zinc finger.
翻訳後修飾RING-type zinc finger-dependent and E2-dependent autoubiquitination.
細胞内局在Cytoplasm > cytosol. The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.