Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 232 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-ZNF318 antibody 画像
Western blot - ZNF318 antibody (ab30972)
Anti-ZNF318 antibody (ab30972) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal stomac lysateSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
232 kDaObserved band size :
>116 kDa (why is the actual band size different from the predicted?
)Additional bands at :
<45 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - ZNF318 antibody (ab30972)
Immunohistochemical analysis of ZNF318 expression in paraffin embedded human pancreas tissue section, using 4 µg/ml ab30972. Arrows indicate positively labelled epithelial cells of pancreatic acinus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ZNF318 antibody(ab30972)
Immunohistochemistry of Human Pancreas lysate tissue at an antibody concentration of 4-8µg/ml using anti-ZNF318 antibody (ab30972)
Anti-ZNF318 antibody (ab30972) 使用論文
has not yet been referenced specifically in any publications.
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