製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1.5625e+006.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    May be involved in transcriptional regulation.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 14 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HZF1 antibody
    • KIAA2007 antibody
    • Zinc finger protein 1 antibody
    • Zinc finger protein 266 antibody
    • Zinc finger protein HZF1 antibody
    • ZN266_HUMAN antibody
    • ZNF 266 antibody
    • ZNF266 antibody
    see all

Anti-ZNF266 antibody 画像

  • Anti-ZNF266 antibody (ab33005) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ZNF266 with ab33005 at 4-8µg/ml. Arrows indicate positively labelled purkinje cells.

  • Anti-ZNF266 antibody (ab33005) at 1/1000 dilution + Lysates were prepared from human HepG2 cells at 25 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is a courtesy of Seth Frietze

    See Abreview

Anti-ZNF266 antibody (ab33005) 使用論文

ab33005 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HepG2 cells)
Loading amount
25 µg
Specification
HepG2 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Seth Frietze

Verified customer

投稿 Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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