製品の概要

  • 製品名Anti-ZNF258 antibody
  • 製品の詳細
    Rabbit polyclonal to ZNF258
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Dog
  • 免疫原

    Synthetic peptide derived from residues 625 - 674 of human ZNF258.

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  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody is purified by peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

Anti-ZNF258 antibody 画像

  • Anti-ZNF258 antibody (ab33004) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 79 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ZNF258 at 4-8µg/ml. Cells with positive label (indicated by arrows) are epithelial cells of renal tubule. Magnification: 400X.

Anti-ZNF258 antibody (ab33004) 使用論文

ab33004 has not yet been referenced specifically in any publications.

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