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KLH conjugated synthetic peptide within central region amino acids 349 - 378 of Human ZNF202.
Our Abpromise guarantee covers the use of ab103034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000. Predicted molecular weight: 75 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/10 - 1/50.|
Immunofluorescence of MDA-MB231 cells labelling ZNF202 with ab103034. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103034 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF202 immunoreactivity is localized to the nucleus.
Immunoflurescence of HepG2 cells labelling ZNF202 with ab103034. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).
Flow cytometry of HepG2 cells (right histogram) labelling ZNF202 with ab103034 compared to a negative control (left histogram). FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
ab103034 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"