製品の概要

  • 製品名Anti-ZNF202 antibody
    ZNF202 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZNF202
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide within central region amino acids 349 - 378 of Human ZNF202.

  • ポジティブ・コントロール
    • WB: HepG2 cell line lysates IHC-P: Human breast carcinoma

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab103034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

ターゲット情報

  • 機能Transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
  • 組織特異性Highly expressed in testis. Also expressed in breast carcinoma cell lines.
  • 配列類似性Contains 8 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
    Contains 1 SCAN box domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Zinc finger protein 202 antibody
    • Zinc finger protein with KRAB and SCAN domains 10 antibody
    • ZKSCAN 10 antibody
    • ZKSCAN10 antibody
    • ZN202_HUMAN antibody
    • ZNF 202 antibody
    • ZNF202 antibody
    see all

Anti-ZNF202 antibody 画像

  • Immunofluorescence of MDA-MB231 cells labelling ZNF202 with ab103034. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103034 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF202 immunoreactivity is localized to the nucleus.

  • Immunoflurescence of HepG2 cells labelling ZNF202 with ab103034. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).

  • Flow cytometry of HepG2 cells (right histogram) labelling ZNF202 with ab103034 compared to a negative control (left histogram). FITC-conjugated Goat anti-rabbit was used as the secondary antibody.

  • Anti-ZNF202 antibody (ab103034) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 75 kDa
  • ab103034, at a 1/50 dilution, staining ZNF202 in formalin fixed and paraffin embedded Human breast carcinoma, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-ZNF202 antibody (ab103034) 使用論文

ab103034 has not yet been referenced specifically in any publications.

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