製品の概要

  • 製品名Anti-ZNF195 antibody
    ZNF195 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZNF195
  • アプリケーション適用あり: WB, ELISA, IHC-P, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog
  • 免疫原

    A region within synthetic peptide: LTFRDVAIEF SLEEWKCLDL AQQNLYRDVM LENYRNLFSV GLTVCKPGLI, corresponding to amino acids 4-53 of Human ZNF195

  • ポジティブ・コントロール
    • WB: Foetal lung lysate IHC-P: Human lung

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性Zinc finger proteins are an important class of eukaryotic DNA binding proteins. The zinc finger motif contains spatially conserved cysteines and histidines, which bind a zinc ion. The most common zinc finger motif is the C2H2 type, which was first identified in the Drosophila segmentation gene, Kruppel. The human genome has several hundred Kruppel related zinc finger genes. About one third of these human Kruppel type genes also code for a highly conserved region, the Kruppel associated box (KRAB domain), comprising approximately 75 amino acids found at the N terminal end. The zinc finger gene ZNF195 contains an N terminal KRAB domain and 14 tandemly repeated Kruppel type zinc finger motifs at its C terminus. ZNF195 may be involved in transcriptional regulation. It is expressed in adult heart, brain, placenta, skeletal muscle and pancreas, and in foetal lung, kidney and brain. There is little expression in adult lung, liver and kidney.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Zinc finger protein 195 antibody
    • ZNFP104 antibody

Anti-ZNF195 antibody 画像

  • Anti-ZNF195 antibody (ab50600) at 1 µg/ml + foetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : ~64 kDa (why is the actual band size different from the predicted?)
  • ab50600 at a concentration of 4-8 µg/ml labelling alveolar cells (labelled with arrows) in paraffin-embedded human lung tissue. Magnification 400X.

Anti-ZNF195 antibody (ab50600) 使用論文

ab50600 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Multiple cell types)
Loading amount 30 µg
Specification Multiple cell types
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Seth Frietze

Verified customer

投稿 Jun 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (Liver carcinoma cell line HepG2)
Total protein in input 30 µg
Specification Liver carcinoma cell line HepG2
Immuno-precipitation step Protein A/G
Username

Dr. Seth Frietze

Verified customer

投稿 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"