製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ZNF148
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (Human) from an internal region

  • ポジティブ・コントロール
    • Human brain tissue HepG2 cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

ターゲット情報

画像

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab69933 at 1/50 dilution. Right panel was treated with immunising peptide.
  • All lanes : Anti-ZNF148 antibody (ab69933) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Gao XH  et al. ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer. Medicine (Baltimore) 96:e5845 (2017). Read more (PubMed: 28072746) »
  • Zhang W  et al. Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer. World J Gastroenterol 21:8836-47 (2015). IHC-P ; Human . Read more (PubMed: 26269673) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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投稿 Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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