Immunohistochemistry (Frozen sections) labeling ZNF133 with ab94754 at 1:100. Tissue was fixed with cold acetone for 10 minutes. rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.
Western blot - ZNF133 antibody (ab94754)
Anti-ZNF133 antibody (ab94754) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 73 kDa Gel concentration: 12%