Anti-ZMPSTE24 抗体 - Carboxyterminal end (ab70952)

製品の概要

  • 製品名
    Anti-ZMPSTE24 antibody - Carboxyterminal end
    ZMPSTE24 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ZMPSTE24 - Carboxyterminal end
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human ZMPSTE24 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and T-47D cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 55 kDa plus a range of other bands. (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Proteolytically removes the C-terminal three residues of farnesylated proteins. Acts on lamin A/C.
    • 組織特異性
      Widely expressed. High levels in kidney, prostate, testis and ovary.
    • 関連疾患
      Defects in ZMPSTE24 are the cause of mandibuloacral dysplasia with type B lipodystrophy (MADB) [MIM:608612]. Mandibuloacral dysplasia (MAD) is a rare autosomal recessive disorder characterized by mandibular and clavicular hypoplasia, acroosteolysis, delayed closure of the cranial suture, joint contractures, and types A or B patterns of lipodystrophy. Type B lipodystrophy observed in MADB, is characterized by generalized fat loss.
      Defects in ZMPSTE24 are a cause of lethal tight skin contracture syndrome (LTSCS) [MIM:275210]; also called restrictive dermopathy (RD). Lethal tight skin contracture syndrome is a rare disorder mainly characterized by intrauterine growth retardation, tight and rigid skin with erosions, prominent superficial vasculature and epidermal hyperkeratosis, facial features (small mouth, small pinched nose and micrognathia), sparse/absent eyelashes and eyebrows, mineralization defects of the skull, thin dysplastic clavicles, pulmonary hypoplasia, multiple joint contractures and an early neonatal lethal course. Liveborn children usually die within the first week of life. The overall prevalence of consanguineous cases suggested an autosomal recessive inheritance.
    • 配列類似性
      Belongs to the peptidase M48A family.
    • 細胞内局在
      Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAAX prenyl protease 1 homolog antibody
      • FACE-1 antibody
      • FACE1 antibody
      • FACE1_HUMAN antibody
      • Farnesylated proteins converting enzyme 1 antibody
      • Farnesylated proteins-converting enzyme 1 antibody
      • Prenyl protein specific endoprotease 1 antibody
      • Prenyl protein-specific endoprotease 1 antibody
      • STE24 antibody
      • Zinc metalloproteinase Ste24 homolog antibody
      • zmpste24 antibody
      see all

    画像

    • Anti-ZMPSTE24 antibody - Carboxyterminal end (ab70952) at 1/100 dilution + T-47D cell lysate at 12.5 µg

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70952 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

    参考文献

    ab70952 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunocytochemistry
    Sample
    Human Cell (umbilical cord blood derived mesenchymal stem cell)
    Permeabilization
    Yes - 0.3% triton X-100
    Specification
    umbilical cord blood derived mesenchymal stem cell
    Blocking step
    normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Username

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    投稿 Oct 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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